The Fall 2023 Biology Seminar Series continues with a talk from Dr. Brandon Taylor, "Enabling high throughput profiling of small molecule splice modulators with RASLseq." The seminar is on Tuesday, November 7th, at 12:00pm.
Small molecule modulation of splicing is a therapeutic modality to disrupt or restore expression of proteins. Although high throughput screening efforts can readily identify splice modulating compounds, optimizing them for splice site specific activity remains a key challenge. Here we present how we utilize RNA-mediated oligonucleotide annealing, selection, and ligation (RASLseq) to examine hundreds of exon junctions simultaneously and measure both on-target activity and specificity for thousands of conditions.